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© 1989 British Society for Rheumatology

research-article

HUMAN CARDIOLIPIN AS THE ANTIGEN IN AN ELISA TO DETECT ANTICARDIOLIPIN ANTIBODIES

R. D. BROWN, J. GIBSON, JENNIFER WELLS, R. JUN, K. RICKARD and H. KRONENBERG

Haematology Department, Royal Prince Alfred Hospital Missenden Road, Camperdown, NSW 2050. Australia

Correspondence to: Correspondence to Ross D. Brown.

Human cardiolipin was purified from cadaver heart tissue and used as the antigen in an enzyme-linked immunoassay (ELISA) for anticardiolipin antibodies. The correlation between assays using human and bovine cardiolipin was r=0.90. Of the lupus anticoagulant positive patients 88% had antibodies to the human antigen and 75% had antibodies to the bovine antigen. Six of 45 samples contained antibodies which only reacted with the human antigen. All six samples remained positive when retested with a different human cardiolipin preparation and were negative when tested against a preparation of sheep cardiolipin. These studies suggest that there was increased specificity when human cardiolipin was used as the phospholipid antigen.

KEY WORDS: Cardiolipin, Human, Enzyme-linked immunosorbent assay, Systemic lupus erythematosus


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