Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by OTTEN, H. G.
Right arrow Articles by BREEDVELD, F. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by OTTEN, H. G.
Right arrow Articles by BREEDVELD, F. C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1989 British Society for Rheumatology

research-article

QUANTITATIVE DETECTION OF CLASS-SPECIFIC RHEUMATOID FACTORS USING MOUSE MONOCLONAL ANTIBODIES AND THE BIOTIN/STREPTAVIDIN ENHANCEMENT SYSTEM

H. G. OTTEN, M. R. DAHA*, H. H. DE ROOIJ and F. C. BREEDVELD

*Department of Nephrology, University Hospital Leiden, The Natherlands
Department of Rheumatology, University Hospital Leiden, The Natherlands

Correspondence to: Correspondence to H. G. Otten, Department of Rheumatology, Building I, C2P, University Hospital, Leden, P.O. Box 9600, 2300 RC Leiden, The Netherlands

An enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of rheumatoid factors (RF) was developed using mouse monoclonal antibodies against human IgG, IgA and IgM together with the biotin/streptavidin enhancement system. One hundred and eight patients with rheumatoid arthritis (RA) of whom 47 had a positive serum latex agglutination assay, 100 healthy controls and 95 diseased controls (25 systemic lupus erythematosus (SLE), 25 ankylosing spondylitis, 20 osteoarthritis and 25 bronchial asthma) were evaluated for the presence of IgG-, IgA- and IgM-RF by ELISA. Elevated levels of IgG-, IgA-, and IgM-RF could be demonstrated in respectively 94%, 91% and 98% of serum samples from RA patients with a positive latex test and in 72%, 69% and 8% of serum samples from RA patients with a negative latex test. In the latter patient group, increased levels of one or more RF isotypes could be detected m 82% of the patients. Except for the presence of IgG-RF in serum of 20% of the SLE patients, increased levels of RF isotypes were present in less than 5% of the diseased controls. Highly significant correlations were found between serum IgM-RF levels as detected by ELISA and those of the latex and the Rose-Waaler agglutination assays. Positive correlations were also found between the serum levels of the three RF isotypes investigated and between the levels of RF isotypes as measured in serum and synovial fluid of the same patient. Compared to agglutination assays, the ELISA for the quantitative detection of RF isotypes is a more reproducible and sensitive test which avoids some of the problems encountered in earlier ELISA methods.

KEY WORDS: Rheumatoid factor, ELISA, Rheumatoid arthritis


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Ann Rheum DisHome page
A E Voskuyl, J M W Hazes, A H Zwinderman, E M Paleolog, F J M van der Meer, M R Daha, and F C Breedveld
Diagnostic strategy for the assessment of rheumatoid vasculitis
Ann Rheum Dis, May 1, 2003; 62(5): 407 - 413.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.