Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by BUCKEE, C.
Right arrow Articles by LENNOX HOLT, P. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by BUCKEE, C.
Right arrow Articles by LENNOX HOLT, P. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1990 British Society for Rheumatology

other

DIVERSITY OF ANTIBODIES TO TYPE II COLLAGEN IN PATIENTS WITH RHEUMATOID ARTHRITIS: DETECTION BY BINDING TO {alpha}-CHAINS AND TO CYANOGEN BROMIDE PEPTIDES

CATHERINE BUCKEE0, K. MORGAN0,1, SHIRLEY AYAD*, I. COLLINS0, R. B. CLAGUE0 and P. J. LENNOX HOLT0

0Departments of Rheumatology Oxford Road, Manchester
*Departments of Biochemistry and Molecular Biology, University of Manchester Medical School Oxford Road, Manchester

Correspondence to: 1Correspondence to Dr K. Morgan, Department of Rheumatology, University of Manchester Medical School, Oxford Road, Manchester M13 9PT.

Antibodies to denatured type II collagen were detected in the sera of a group of patients with rheumatoid arthritis by ELISA and by immunoblotting.

The antibodies were further examined by immunoblotting against cyanogen-bromide derived peptides of type II collagen. The majority of sera reacted against only one or two peptides and antibodies to the CB-l0 and CB-11 peptides were those most commonly found. However, some sera reacted with up to eight peptides, indicating that patients had antibodies to differing combinations of epitopes on type II collagen.

Examination of sequential serum samples from an individual patient showed that there were changes in the class of antibody produced to type II collagen and that antibodies to different peptides were preferentially produced at different times in the course of the disease.

Thus there was a selective response to different peptides of type II collagen not only between patients but also at different times in the course of disease in the same patient.

KEY WORDS: Antibodies, Type XI collagen, Type II collagen, Western blotting, ELISA, Sequential results


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Ann Rheum DisHome page
M. Mullazehi, L. Mathsson, J. Lampa, and J. Ronnelid
High anti-collagen type-II antibody levels and induction of proinflammatory cytokines by anti-collagen antibody-containing immune complexes in vitro characterise a distinct rheumatoid arthritis phenotype associated with acute inflammation at the time of disease onset
Ann Rheum Dis, April 1, 2007; 66(4): 537 - 541.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.