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© 1992 British Society for Rheumatology

research-article

MEASUREMENT OF HUMAN PHOSPHOLIPASE A IN ARTHRITIS PLASMA USING A NEWLY DEVELOPED SANDWICH ELISA

G. M. SMITH, R. L. WARD*, L. MCGUIGAN{dagger}, I. A. RAJKOVIC and K. F. SCOTT

Pacific Biotechnology Ltd 74 McLachlan Avenue, Rushcutters Bay, NSW 2011, Australia
*Department of Haematology, St Vincenis Hospital Victoria Street, Darlinghurst, NSW 2010, Australia
{dagger}Department of Rheumatology, St George Hospital Kogarah, NSW 2217, Australia

Correspondence to: Correspondence to G. Smith, Department of Haematology, St Vincents Hospital, Victoria Street, Darlinghurst, NSW 2010, Australia

A sandwich enzyme-linked immunosorbent assay (ELISA) for human non-pancreatic phospholipase A2 (PLA2) was developed using monoclonal antibodies raised against purified recombinant PLA2 This assay was shown to be specific for human non-pancreatic PLA2 showing no cross-reactivity with human pancreatic PLA2 or with snake venom PLA2 (Crotalus durissus). The immunoassay showed no cross-reactivity with plasma components, and was reproducible and quantitative between 39 pmol and 2·7 nmol PLA2/l The levels of non-pancreatic PLA2 in the plasma of patients with arthritis was measured using this immunoassay. There were significantly higher levels of PLA2 in patients with rheumatoid arthritis than in those with osteoarthritis or healthy controls. Plasma PLA2 was highest in those patients with active rheumatoid arthritis.

KEY WORDS: Human non-pancreatic phospholipase A2, Sandwich ELISA, Rheumatoid arthritis


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