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© 1993 British Society for Rheumatology

research-article

A QUANTITATIVE ELISA FOR MEASUREMENT OF RHEUMATOID FACTOR ASSOCIATED CROSS-REACTIVE IDIOTYPES IN SERUM FROM PATIENTS WITH RHEUMATIC DISEASES

F. SHOKRI*, R. A. MAGEED{dagger} and R. JEFFERIS

Department of Immunology, The Medical School, University of Birmingham Edgbaston, Birmingham B15 2TT

Correspondence to: Correspondence to: R. Jefferis

A sensitive ELISA for the quantitation of RF associated cross-reactive idiotypes (CRI) has been developed. CRI bearing IgM was captured by immobilized F(ab')2 fragments of monoclonal anti-CRI antibodies and revealed with isotype specific conjugates. Factors affecting sensitivity, specificity and reproducibility of the assay were assessed. The sensitivity limit was less than 10 ng/ml and the linear assay range for the CRI standard curve was from 80 to 500 ng/ml. Using this ELISA system we have quantitated serum levels of the CRI in different groups of patients with RA, early synovitis and normal individuals. Our results indicate a significant increase in the levelsof the CRI in the patient groups compared to the normal subjects tested, and suggest involvement of a diverse repertoire of Ig variable region germline genes and/or somatic mutation in production of polyclonal RF in RA.

KEY WORDS: ELISA, Rheumatoid factor, Rheumatoid arthritis, Cross-reactive idiotypes

*Present address: Department of Immunology, School of Public Health, University of Medical Sciences of Tehran, Tehran, Iran

{dagger}Present address: The Kennedy Institute of Rheumatology, London


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