© 1994 British Society for Rheumatology
research-article |
DETECTION OF AUTOANTIBODIES TO THE 90 kDa HEAD SHOCK PROTEIN IN SYSTEMIC LUPUS ERYTHEMATOSUS AND OTHER AUTOIMMUNE DISEASES
Medical Molecular Biology Unit, Department of Molecular Pathology 46 Cleveland St, London W1P 6DP
*Bloomsbury Rheumatology Unit, Department of Medicine UCL Medical School, London
Correspondence to:
Correspondence to: D. A. Isenberg, Bloomsbury Rheumatology Unit, Arthur Stanley House, Tottenham Street, London W1P 9PG.
Expression of the highly conserved 90 kDa heat shock protein (Hsp90) is elevated in the peripheral blood mononuclear cells of approximately 25% of patients with SLE. Conflicting data have been published about the frequency of antibodies to Hsp90 with the previous methodology using a complex Western blot system. We now describe an ELISA to measure autoantibodies to Hsp90 and Hsp70 in SLE patients, healthy controls and patients with a variety of autoimmune rheumatic diseases, IgG and IgM antibodies were elevated in 26 and 35% of SLE patients, respectively. These results show autoantibodies to Hsp90 (but not Hsp70) are elevated in a significant proportion of patients with SLE (P<0.025) compared to healthy controls; and that those with raised antibody levels were more likely to have renal disease and a low C3 level (P<0.02).
KEY WORDS: Heat shock protein 90, Autoantibodies, Systemic lupus erythematosus, Autoimmunity, Myositis
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