© 1994 British Society for Rheumatology
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HIGH FREE AND LATENT COLLAGENASE ACTIVITY IN PSORIATIC ARTHRITIS SYNOVIAL FLUIDS
*Ludwig Boltzmann Institute of Rheumatology and Balneology A-1107 Vienna-Oberlaa, Austria;
2nd Medical Department, Center for Diagnosis and Therapy of Rheumatic Diseases, Hospital Lainz A-1130 Vienna, Austria;
National Institute of Rheumatology and Physiotherapy (ORFI) Budapest, Hungary;
5th Medical Department (Rheumatology and Rehabilitation)Wilhelminenspital A-1160 Vienna, Austria
||2nd Medical Department (Rheumatology and Osteology), Kaiser Franz Josef-Hospital A-1100 Vienna, Austria;
¶Orthopaedic Department, Pulmological Center of the City of Vienna A-1145 Vienna, Austria;
**Institute of Internal Medicine, University of Cagliari Italy
*University of Cagliari Italy
Collagenase activity has been studied intensively in SF from OA and RA patients. Less is known about collagenolytic activity in PsA SF. Therefore we examined collagenolytic activity in crude and trypsin treated SF as well as the 
l-antitrypsin and 2-macroglobulin concentrations in 50 patients suffering from OA (n = 13), RA (n = 17), and PsA (n = 20). Free collagenolytic activity was low in the crude OA SF (1.80 ± 1.35 µg released collagen/min/ml SF) and almost equally low in RA SF (2.35 ± 1.80 µg released collagen/min/ml SF; P>0.3). The PsA SF, however, exhibited a significantly higher free collagenolytic activity (5.63 ± 5.69 µg released collagen/min/ml SF; P<0.05 in comparison to OA and RA SF). The treatment of the SF with trypsin further activated collagenolytic activity in each group (OA 2.17 ± 135 µg released collagen/min/ml SF; RA 6.48 ± 6.73 µg released collagen/min/ml SF; PsA 11.24 ± 5.02 µg released collagen/min/ml SF) and yielded significant differences between OA and RA, OA and PsA, and RA and PsA SF (P < 0.05). Concomitantly with the collagenolytic activity, the l-antitrypsin and 2-macroglobulin concentrations of the SF were measured. In SF from patients with PsA (172.9 ± 69.4 mg/100 ml) and RA (190.6 ± 64.7 mg/100 ml) the l-antitrypsin was significantly higher than in those from OA SF (106.1 ± 39.2 mg/100 ml). On the other hand the 2-macroglobulin concentration was significantly lower in PsA SF (70.5 ± 17.2 mg/100 ml) in comparison with OA (91.9 ± 323 mg/100 ml; P < 0.03) and RA (96.4 ± 35.0 mg/100 ml P < 0.05) SF. The results of the present study show that in contrast to RA and OA SF, PsA SF contain an unexpectedly high free collagenolytic activity which is significantly correlated with the low 2-macroglobulin concentration in these SF
KEY WORDS: Synovial fluid, Collagenase activity, Inflammatory disease, Psoriatic arthritis.
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