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© 1995 British Society for Rheumatology

research-article

FREQUENCY OF AUTOANTIBODIES TO A MAJOR EPITOPE ON THE CARBOXYL TERMINAL FRAGMENT OF CENP-B IN PATIENTS WITH AUTOIMMUNE DISEASE

J. WHYTE*, E. SORIANO*,{dagger},§, W. C. EARNSHAW{ddagger} and N. J. McHUGH*,{dagger},

*Bath Institute for Rheumatic Diseases Bath
{dagger}Royal National Hospital for Rheumatic Diseases Bath
{ddagger}Department of Cell Biology and Anatomy, Johns Hopkins University Baltimore, Maryland, USA

Correspondence to: Correspondence to: N. J. McHugb, Royal National Hospital for Rheumatic Diseases, Upper Borough Walls, Bath BA1 1RL.

The carboxyl-terminal fragment of CENP-B contains a major epitope for anti-centromere antibodies (ACA). We have developed an enzyme-linked immunoassay (ELISA) for measuring antibodies to the 147-carboxyl-terminal amino acids of CENP-B expressed as a ß-galactosidase fusion protein. The ELISA was 98% sensitive and 95% specific for detecting ACA in a population which included 46 patients with ACA detected by other means. Therefore, the CENP-B ELISA should prove avaluable tool in screening for ACA in populations at risk of developing systemic sclerosis, such as those with Raynaud's phenomenon. Levels of anti-CENP-B antibodies were not increased in unaffected relatives of probands with ACA.

KEY WORDS: Scleroderma, Anti-centromere antibody, Autoantigen

§Current address: Rheumatology Unit, Medical Services, Hospital Italiano de Buenos Aires, Argentina.


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