© 1996 British Society for Rheumatology
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EXPRESSION OF THE MULTDORUG RESISTANCE GLYCOPROTEIN 170 IN THE PERIPHERAL BLOOD LYMPHOCYTES OF RHEUMATOID ARTHRITIS PATIENTS. THE PERCENTAGE OF LYMPHOCYTES EXPRESSING GLYCOPROTEIN 170 IS INCREASED IN PATIENTS TREATED WITH PREDNISOLONE
*Laboratoire d'Himatologie CHU Dijon, France
Service de Rhumatologie, Centre Hospitalier Lyon-Sud France
Service d'Epidimiologie et Hygiène Hospitalière CHU Dijon, France
Laboratoire de Biophysique, Facultè de Mèdecine Dijon, France
Service de Rhunatologie France
Correspondence to:
Correspondence to: J. F. Maillefert, Service de Rhumatologie, Hôpital Général, 3 rue du Fb Raines, 21 000 Dijon, France.
The objective was to evaluate the expression of the multidrug resistance P-glycoprotein (P-gp) in peripheral blood lymphocytes (PBL) of patients with rheumatoid arthritis (RA). PBL from 68 RA patients and 44 controls were evaluated. RA patients had a mean disease duration of 10.7 yr, with a mean number of past resistances to DMARDs of 0.82, and were treated with NSAIDs (n = 34), DMARDs (n = 25) and prednisolone (n = 40). Fluorescence flow cytometry was used to assess P-gp membrane expression on PBL. In the RA group, the percentage of PBL expressing P-gp was Higher in patients treated with prednisolone than in other patients [mean ± s.D.: 10.7 ± 15.8% vs 3.3 ± 7.6%, P < 0.03, Student] and was not related to other therapies, age, sex, RA duration, number of past resistances to DMARDs, activity, ESR, CRP. The percentage of PBL expressing P-gp did not differ in RA and control groups, but was higher in the prednisolone-treated RA patients than in controls. Prednisolone could induce a rise in the percentage of PBL expressing P-gp. On the contrary, patients with a high percentage of PBL expressing P-gp could be more resistant to DMARDs and need prednisolone earlier. Further studies are needed to address this question and to evaluate the potential implication of P-gp in drug resistance in RA.
KEY WORDS: Rheumatoid arthritis, Treatment, MDR, Drug, Resistance, Glucocorticoids
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