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© 1996 British Society for Rheumatology

research-article

TYROSINE PHOSPHORYLATION IN NEUTROPHILS FROM SYNOVIAL FLUID OF PATIENTS WITH RHEUMATOID ARTHRITIS

D. LLOYDS, E. V. DAVIES, B. D. WILLIAMS* and M. B. HALLETT

Departments of Surgery Heath Park, Cardiff CF4 4XN
*Rheumatology, University of Wales College of Medicine Heath Park, Cardiff CF4 4XN

Correspondence to: Correspondence to: M. B. Hallett, Molecular Signalling Group, Department of Surgery, University of Wales College of Medicine, Heath Park, Cardiff CF4 4XN

The priming of neutrophils is associated with an increase in the level of tyrosine phosphorylation of cytosolic proteins, specifically of proteins with mol. wts of 42 and 74 kDa. We show here, using dot blots and Western blotting, that neutrophils isolated from rheumatoid synovial fluid have increased tyrosine phosphorylation of these target proteins. The level of tyrosine phosphorylation within neutrophils in the synovial fluid was increased when compared with neutrophils from the blood of the same patients, normal blood or neutrophils from the synovial fluid of patients without rheumatoid arthritis. Neutrophils from the rheumatoid synovial fluid were also more active and were unable to be further primed by exogenous primers. These data suggest that this elevation of tyrosine phosphorylation was the result of the action of local priming agents within the rheumatoid synovial fluid.

KEY WORDS: Neutrophils, Rheumatoid arthritis, Tyrosine phosphorylation, priming


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