The British Journal of Rheumatology, Vol 37, 324-330, Copyright © 1998 by British Society for Rheumatology
RJ Dolhain, NT Ter Haar, R De Kuiper, IG Nieuwenhuis, AH Zwinderman, FC Breedveld and AM Miltenburg
A prerequisite for comparative histology of synovial tissue by means of
biopsies is insight into the distribution of a marker under study. This
investigation focuses on the variation in the presence of T cells and signs
of T-cell activation within the rheumatoid joint. For this purpose,
multiple slides from several pieces of synovial tissue from different parts
of a joint were stained and scored for the expression of CD3, CD25, HLA-DR,
Ki67 and interferon-gamma. The variation in scores for the presence of T
cells and markers of activation was more pronounced in slides prepared from
different pieces of tissue than in slides from one piece of tissue. Based
on multiple analysis of variance, methods are suggested to establish a
reliable overall score for the expression of a certain marker within a
joint. Following validation, such methods may prove to be useful by
allowing semiquantitative histology of synovial tissue for studies on
arthritis.
ORIGINAL PAPERS
Distribution of T cells and signs of T-cell activation in the rheumatoid joint: implications for semiquantitative comparative histology
Department of Rheumatology, Leiden University Hospital, The Netherlands.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A W R van Kuijk, D M Gerlag, K Vos, G Wolbink, M de Groot, M A de Rie, A H Zwinderman, B A C Dijkmans, and P P Tak A prospective, randomised, placebo-controlled study to identify biomarkers associated with active treatment in psoriatic arthritis: effects of adalimumab treatment on synovial tissue Ann Rheum Dis, August 1, 2009; 68(8): 1303 - 1309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R M Thurlings, K Vos, C A Wijbrandts, A H Zwinderman, D M Gerlag, and P P Tak Synovial tissue response to rituximab: mechanism of action and identification of biomarkers of response Ann Rheum Dis, July 1, 2008; 67(7): 917 - 925. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Rooney, B. Bresnihan, U. Andersson, M. Gogarty, M. Kraan, H R. Schumacher, A.-K. Ulfgren, D. J Veale, P. P Youssef, and P. P Tak Microscopic measurement of inflammation in synovial tissue: inter-observer agreement for manual quantitative, semiquantitative and computerised digital image analysis Ann Rheum Dis, December 1, 2007; 66(12): 1656 - 1660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M Gerlag, D. L Boyle, S. Rosengren, T. Nash, P. P Tak, and G. S Firestein Real-time quantitative PCR to detect changes in synovial gene expression in rheumatoid arthritis after corticosteroid treatment Ann Rheum Dis, April 1, 2007; 66(4): 545 - 547. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Gregorio, C. Gambini, V. Gerloni, A. Parafioriti, M. P. Sormani, S. Gregorio, G. De Marco, F. Rossi, A. Martini, and M. Gattorno Lymphoid neogenesis in juvenile idiopathic arthritis correlates with ANA positivity and plasma cells infiltration Rheumatology, February 1, 2007; 46(2): 308 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z N Jahangier, J W G Jacobs, M C Kraan, M J G Wenting, T J Smeets, J W J Bijlsma, F P J G Lafeber, and P P Tak Pretreatment macrophage infiltration of the synovium predicts the clinical effect of both radiation synovectomy and intra-articular glucocorticoids Ann Rheum Dis, October 1, 2006; 65(10): 1286 - 1292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. A. Kroot, A. E. A. M. Weel, J. M. W. Hazes, P. E. Zondervan, M. P. Heijboer, P. L. A. van Daele, and R. J. E. M. Dolhain Diagnostic value of blind synovial biopsy in clinical practice Rheumatology, February 1, 2006; 45(2): 192 - 195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M D Smith, D Baeten, A-K Ulfgren, I B McInnes, O Fitzgerald, B Bresnihan, P P Tak, D Veale, and on behalf of the OMERACT synovial special interest Standardisation of synovial tissue infiltrate analysis: how far have we come? how much further do we need to go? Ann Rheum Dis, January 1, 2006; 65(1): 93 - 100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R J Verburg, R Flierman, J K Sont, F Ponchel, L van Dreunen, E W Levarht, M M Welling, R E M Toes, J D Isaacs, and J M van Laar Outcome of intensive immunosuppression and autologous stem cell transplantation in patients with severe rheumatoid arthritis is associated with the composition of synovial T cell infiltration Ann Rheum Dis, October 1, 2005; 64(10): 1397 - 1405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M J Benito, D J Veale, O FitzGerald, W B van den Berg, and B Bresnihan Synovial tissue inflammation in early and late osteoarthritis Ann Rheum Dis, September 1, 2005; 64(9): 1263 - 1267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J M Koski and M Helle Ultrasound guided synovial biopsy using portal and forceps Ann Rheum Dis, June 1, 2005; 64(6): 926 - 929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M C Kraan, J J Haringman, H Weedon, E C Barg, M D Smith, M J Ahern, T J M Smeets, F C Breedveld, and P P Tak T cells, fibroblast-like synoviocytes, and granzyme B+ cytotoxic cells are associated with joint damage in patients with recent onset rheumatoid arthritis Ann Rheum Dis, May 1, 2004; 63(5): 483 - 488. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Rosengren, G. S. Firestein, and D. L. Boyle Measurement of Inflammatory Biomarkers in Synovial Tissue Extracts by Enzyme-Linked Immunosorbent Assay Clin. Vaccine Immunol., November 1, 2003; 10(6): 1002 - 1010. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. P. Tak Lessons learnt from the synovial tissue response to anti-rheumatic treatment Rheumatology, August 1, 2000; 39(8): 817 - 820. [Full Text] [PDF]
|
|
|
|
|
|
B. Bresnihan, P. P. Tak, P. Emery, L. Klareskog, and F. Breedveld Synovial biopsy in arthritis research: five years of concerted European collaboration Ann Rheum Dis, July 1, 2000; 59(7): 506 - 511. [Full Text]
|
|