The British Journal of Rheumatology, Vol 37, 562-569, Copyright © 1998 by British Society for Rheumatology
K Muller, EB Herner, A Stagg, K Bendtzen and P Woo
In the present study, we investigated the kinetics and the activation
thresholds for the production of a number of pro-inflammatory cytokines and
cytokine antagonists in Escherichia coli lipopolysaccharide (LPS) or
phytohaemagglutinin (PHA) stimulated whole blood cultures of 13 patients
with systemic juvenile chronic arthritis (SJCA) and 10 healthy children. In
unstimulated cultures, the levels of interleukin (IL)- 1beta, IL-6 and
tumour necrosis factor alpha (TNF-alpha) were undetectable in both groups,
suggesting that there was no spontaneous production of these cytokines by
circulating leucocytes. The activation thresholds for the production of
these cytokines, as well as the capacity for production, did not differ
significantly between patients and controls. The level of interleukin-1
receptor antagonist (IL-1ra) in plasma of the patients was significantly
elevated, while the in vitro production of IL-1ra was essentially normal
and it did not correlate with plasma levels of IL-1ra. Supernatant levels
of soluble TNF-alpha receptor (sTNF-R) I and II were both significantly
elevated and correlated with the global activity score. In contrast, the
supernatant levels of IL-10 were reduced in both PHA- and LPS-driven
cultures. Although IL-10 levels did not correlate with laboratory or
clinical indices of disease activity, the results suggest that reduced
IL-10 production may play a pathogenetic role in SJCA.
ORIGINAL PAPERS
Inflammatory cytokines and cytokine antagonists in whole blood cultures of patients with systemic juvenile chronic arthritis
Institute of Inflammation Research, Paediatric Department, Rigshospitalet National University Hospital, Copenhagen, Denmark.
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