The British Journal of Rheumatology, Vol 37, 773-778, Copyright © 1998 by British Society for Rheumatology
MA Iniguez, JL Pablos, PE Carreira, F Cabre and JJ Gomez-Reino
OBJECTIVE: To investigate the expression of cyclooxygenase-1 (COX-1) and
cyclooxygenase-2 (COX-2) in cells from synovial fluid (SF) of patients with
acute or chronic arthritis. METHODS: SF was obtained from eight patients
with acute crystal-induced arthritis, nine with rheumatoid arthritis and
four with psoriatic arthritis. COX-1 and COX-2 gene expression was studied
by reverse transcriptase-polymerase chain reaction (RT-PCR). Protein
expression was detected by Western blotting and immunocytochemistry.
RESULTS: There was expression of COX-1 mRNA in all and COX-2 mRNA in most
of the SF samples from acute or chronic arthritis. By immunocytochemistry,
both COX-1 and COX-2 immunoreactivity was restricted to a variable fraction
of mononuclear cells. COX-1 staining was observed in 10-fold more cells
than COX-2. By Western blotting, COX-1 protein was detected in 60% of the
SF samples and COX-2 in none. There were no differences in the pattern of
COX-1 and COX-2 expression between chronic and acute SF samples.
CONCLUSION: In arthritis, both COX-1 and COX-2 isoforms are expressed by SF
cells. COX-1 is the most abundant isoform. Since the strong COX-1
immunostaining observed in a fraction of mononuclear SF cells is not
observed in peripheral blood leucocytes, it may be the result of either the
activation or recruitment of a subset of mononuclear cells with a high
COX-1 expression level.
ORIGINAL PAPERS
Detection of COX-1 and COX-2 isoforms in synovial fluid cells from inflammatory joint diseases
Servicio de Reumatologia y Unidad de Investigacion, Hospital 12 de Octubre, Madrid, Spain.
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