The British Journal of Rheumatology, Vol 37, 1003-1007, Copyright © 1998 by British Society for Rheumatology
PP Youssef, TJ Smeets, B Bresnihan, G Cunnane, O Fitzgerald, F Breedveld and PP Tak
Microscopic measurement of inflammation in synovial tissue may be important
in studies of clinical status, prognosis and response to treatment. The aim
of this study was to compare quantitative microscopic analysis of
inflammation with a semiquantitative grading system in rheumatoid arthritis
(RA) synovial membrane. Knee synovial membrane samples from 16 patients
with RA, including paired samples taken before and after treatment in nine
patients, were immunostained with anti-CD68 and anti-CD3 monoclonal
antibodies using standard techniques. The intensity of macrophage and
T-lymphocyte infiltration was measured both by quantitative and
semiquantitative techniques, and the results were compared. In a
cross-sectional comparison, both methods correlated significantly for
lining layer macrophage infiltration, as well as sublining layer macrophage
and T-cell infiltration. However, in some patients demonstrating a clinical
response to treatment, semiquantitative analysis lacked sensitivity to
biologically relevant changes in mononuclear cell infiltration. These
observations have important implications for future studies of therapeutic
modalities.
ORIGINAL PAPERS
Microscopic measurement of cellular infiltration in the rheumatoid arthritis synovial membrane: a comparison of semiquantitative and quantitative analysis
University College Dublin Department of Rheumatology, St Vincent's Hospital, Ireland.
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