Rheumatology 1999; 38: 751-754
© 1999 British Society for Rheumatology
Paediatric Rheumatology: Autologous Stem Cell Transplantation in Rheumatic Diseases of Childhood |
The depletion of T cells from haematopoietic stem cell transplants
Series Editor: P. Woo
CLB, Sanquin Blood Supply Foundation, Department of Transfusion Technology, Stem Cell Laboratory, Laboratory for Experimental and Clinical Immunology, Academic Medical Centre, University of Amsterdam,
1 Department of Internal Medicine, Academic Medical Centre, Amsterdam,
2 Department of Haematology, University Hospital Utrecht, Utrecht,
3 Department of Paediatric Immunology, University Hospital for Children, `het Wilhelmina Kinderziekenhuis', Utrecht and
4 Department of Paediatrics, University Hospital Leiden, Leiden,The Netherlands
Correspondence to:
I. C. M. Slaper-Cortenbach, CLB Sanquin Blood Supply Foundation, Department of Transfusion Technology, Stem Cell Laboratory, PO Box 9190, 1006 AD Amsterdam, The Netherlands.
Abstract
Objective. In our laboratory, we have developed an immunorosette technique for the depletion of T cells from bone marrow transplants. Tetrameric complexes of monoclonal antibodies are able to form very stable immunorosettes, which are efficiently depleted with the aid of a blood cell separator. Major improvements over the original sheep red blood cell depletion are the use of human (patient or donor derived) erythrocytes instead of sheep-derived cells, and the possibility of using a closed system for separation in a cell separator. In contrast to bone marrow, mobilized haematopoietic stem cell transplants obtained after leucocytapheresis contain higher numbers of T cells. Therefore, a different approach is necessary.
Method. We have used two CD34 selection systems (IsolexTM 300SA and the ClinimacsTM) to perform T-cell depletions from peripheral blood stem cell (PBSC) transplants.
Results. Immunorosette T-cell depletion, with CD2/CD3 tetrameric complexes, of bone marrow transplants resulted in a mean 2.5 log depletion of T cells with a yield of 50% of the CD34+ cell population. Stem cell selection of PBSC transplants using one of the CD34 selection procedures resulted in a 4.5 log depletion of T cells for both systems, but with different results for the recovery of CD34+ cells. An increased yield of CD34+ cells was obtained with the ClinimacsTM procedure (57.9±9.0%) in comparison to the IsolexTM procedure (40.1±12.5%).
Conclusion. Our own immunorosette depletion technique and the two tested CD34 selection methods for stem cell transplants both resulted in a very efficient T-cell depletion with the recovery of 40–60% of the CD34+ haematopoietic stem cells present in the transplant.
KEY WORDS: T cells, Depletion, Haematopoietic stem cell transplants.
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