Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (14)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Kacem, H. H.
Right arrow Articles by Ayadi, H.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Kacem, H. H.
Right arrow Articles by Ayadi, H.
Related Collections
Right arrow Immunogenetics
Right arrow Sjogren's Syndrome
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Rheumatology 2001; 40: 1370-1374
© 2001 British Society for Rheumatology

Original Papers

HLA-DQB1 CAR1/CAR2, TNFa IR2/IR4 and CTLA-4 polymorphisms in Tunisian patients with rheumatoid arthritis and Sjögren's syndrome

H. Hadj Kacem, N. Kaddour1, F.-Z. Adyel, Z. Bahloul1 and H. Ayadi

Laboratoire de Génétique Moléculaire Humaine, Faculté de médecine, Sfax and
1 Service de médecine interne CHU Hédi Chaker, Sfax, Tunisia

Objectives. To evaluate the contribution of HLA class II region and the CTLA-4 gene in genetic susceptibility to rheumatoid arthritis (RA) and Sjögren's syndrome (SS) in the Tunisian population.

Methods. The polymorphisms of a (CA)n microsatellite of HLA-DQB1 CAR1/CAR2, TNFa IR2/IR4 and an (AT)n microsatellite in the 3'-untranslated region of exon 3 of the CTLA-4 gene were analysed after specific polymerase chain reaction (PCR) amplification. Typing of CTLA-4 A/G exon 1 polymorphism was achieved by the PCR–restriction fragment length polymorphism method.

Results. Genomic DNA from 60 patients with RA, 58 patients with SS and 150 healthy individuals was genotyped. The distribution of HLA-DQ CAR1/CAR2 allele frequencies differed between patients and controls in both diseases (RA, P<10-15; SS, P=7.6x10-15; RA+SS, P<10-15). The analysis of TNFa IR2/IR4 and CTLA-4 A/G polymorphisms did not show any differences in allele or genotype frequencies between patients and control subjects in either disease. The distribution of CTLA-4 (AT)n allele frequencies differed between patients with RA and controls (P=10-3), whereas no significant difference was detected between patients with SS and controls.

Conclusion. These data suggest the involvement of HLA-DQ CAR1/CAR2 polymorphisms in genetic susceptibility to RA and SS and the participation of the CTLA-4 gene, or a gene closely associated with it, in the development of RA.

KEY WORDS: HLA-DQ CAR, TNF, CTLA-4, Association, Polymorphism, Rheumatoid arthritis, Sjögren's syndrome.

Correspondence to: H. Ayadi, Faculté de Médecine, Laboratoire de Génétique Moléculaire Humaine, Av. Majida Boulila 3029, Sfax, Tunisia


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Rheumatology (Oxford)Home page
M. Ramos-Casals and J. Font
Primary Sjogren's syndrome: current and emergent aetiopathogenic concepts
Rheumatology, November 1, 2005; 44(11): 1354 - 1367.
[Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.