Rheumatology 2001; 40: 673-682
© 2001 British Society for Rheumatology
Original Papers |
Osteoclast-like cells in an in vitro model of bone destruction by rheumatoid synovium
Department of Rheumatology,
1 Institute of Medical Science and
2 Department of Orthopedic Surgery, St Marianna University School of Medicine, Kawasaki and
3 Department of Pathology, Tokai University School of Medicine, Isehara, Japan
Objective. Osteoclasts may be involved in the process of rheumatoid bone destruction. To test this hypothesis, we developed an in vitro model of bone destruction by osteoclast-like cells derived from cultured rheumatoid synovial tissue without using any inducers.
Methods. Synovial tissues were obtained from rheumatoid arthritis and osteoarthritis patients and tissue pieces of about 2 mm3 that contained synovial lining were cultured. Multinucleated cells derived from cultured synovial tissues were studied cytochemically and morphologically for osteoclast-specific markers.
Results. Fibroblast-like and macrophage-like cells from the tissue pieces proliferated in the coexistence of lymphocytes. After 14 days of culture, multinucleated cells with tartrate-resistant acid phosphatase activity appeared. These cells expressed vacuolar H+-ATPase, the vitronectin receptor and cathepsin K. Although binding of 125I-labelled salmon calcitonin was very low, the cells contained ringed structures of F-actin and showed strong bone-resorbing activity on ivory slices. Proliferation of macrophage-like cells and formation of multinucleated cells continued during 6 months of culture in the presence of fibroblast-like cells. The bone-resorbing activity of multinucleated cells derived from rheumatoid synovial tissue was much higher than that of cells from osteoarthritis synovial tissue, and was related to the disease activity of rheumatoid arthritis.
Conclusion. Our culture system reproduced in vitro the process of bone destruction by rheumatoid synovium, including the proliferation and fusion of precursor cells, polarization, activation and bone tissue resorption. This system may provide a tool for understanding the mechanisms of bone destruction in rheumatoid arthritis and for the development of new therapies to prevent bone destruction.
KEY WORDS: Rheumatoid arthritis, Bone destruction, Osteoclasts, Synovial tissue macrophages, In vitro model.
Correspondence to: Y. Suzuki, Department of Rheumatology, St Marianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki 216-8512, Japan.
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