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Rheumatology 2002; 41: 759-766
© 2002 British Society for Rheumatology

Original Papers

A 588-gene microarray analysis of the peripheral blood mononuclear cells of spondyloarthropathy patients

J. Gu, E. Märker-Hermann3, D. Baeten5, W. C. Tsai6, D. Gladman7, M. Xiong1, H. Deister3, J. G. Kuipers4, F. Huang9, Y. W. Song10, W. Maksymowych8, J. Kalsi, M. Bannai11, N. Seta, M. Rihl, L. J. Crofford2, E. Veys5, F. De Keyser5 and D. T. Y. Yu

University of California at Los Angeles, CA,
1 University of Texas at Houston, Houston, TX and
2 University of Michigan, Ann Arbor, MI, USA,
3 University of Mainz, Mainz and
4 Hannover Medical School, Hannover, Germany,
5 University of Ghent, Ghent, Belgium,
6 Kaoshiung Medical College, Kaoshiung, Republic of China,
7 University of Toronto, Toronto and
8 University of Alberta, Edmonton, Canada,
9 PLA General Hospital, Beijing, China,
10 Seoul National University, Seoul, Korea and
11 Japanese Red Cross Tokyo Metropolitan Blood Center, Tokyo, Japan

Objectives. To identify genes which are more highly expressed in the peripheral blood mononuclear cells (PBMC) of patients with spondyloarthropathy (SpA), rheumatoid arthritis (RA) and psoriatic arthritis (PsA), in comparison to normal subjects.

Methods. A 588-gene microarray was used as a screening tool to select a panel of such genes from PBMC of these subjects and of normal subjects. Results were then validated by reverse transcription–polymerase chain reaction (RT-PCR).

Results. The following genes were more highly expressed in arthritis patients than in normal subjects: macrophage differentiation marker MNDA (myeloid nuclear differentiation antigen), MRP8 and MRP14 (migratory inhibitory factor-related proteins); signalling molecules JAK3 (janus kinase 3) and MAP kinase p38 (mitogen-activated protein kinase); receptors TNFR2/p75, C-C-chemokine receptor type 1 (CCR1), C-X-C-chemokine receptor type 4 (CXCR4) and integrin ß1; and the cytokines/chemokines interleukin (IL) 1ß and IL-8. Expression of CXCR4 was unexpectedly high among all arthritis subjects. Using RT-PCR, ELISA and immunohistology, expression of stromal cell-derived factor 1 (SDF-1) was demonstrated in arthritis joints.

Conclusions. The CXCR4/SDF-1 is a potential pro-inflammatory axis for RA, PsA and SpA.

KEY WORDS: Microarray, Spondyloarthropathy, Rheumatoid arthritis, Spondyloarthropathy.

Correspondence to: D. Yu, Rheumatology Division, 35–40 Rehabilitation Center, UCLA, 1000 Veteran Avenue, Los Angeles, CA 90095–167022, USA.


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