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Rheumatology Advance Access originally published online on June 16, 2003
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Rheumatology 2003; 42: 1306-1310
© 2003 British Society for Rheumatology

Ciprofloxacin reduces the stimulation of prostaglandin E2 output by interleukin-1ß in human tendon-derived cells

A. N. Corps, V. A. Curry, R. L. Harrall, D. Dutt, B. L. Hazleman and G. P. Riley

Rheumatology Research Unit, Addenbrooke's Hospital, Cambridge, UK.

Correspondence to: A. N. Corps, Rheumatology Research Unit, Box 194, Unit E6, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QQ, UK.

Objective. Fluoroquinolone antibiotics such as ciprofloxacin can induce tendon pathology and have various effects on tendon-derived cells in culture. We are investigating whether ciprofloxacin modifies signalling responses in tendon cells.

Methods. Human Achilles tendon-derived cells were preincubated with or without ciprofloxacin (50 µg/ml) and were then challenged with interleukin-1ß (IL-1ß, 1 ng/ml) for up to 48 h. Prostaglandin E2 (PGE2) output was assayed by ELISA. The expression of cyclooxygenase-2 (COX-2) was examined by Western blotting.

Results. IL-1ß stimulated a substantial and prolonged increase in the output of PGE2. Preincubation with ciprofloxacin reduced IL-1ß-induced PGE2 output at all times tested; the reduction at 48 h was 69% (99% confidence interval 59–79%; 15 experiments). Norfloxacin and ofloxacin also reduced PGE2 output. However, ciprofloxacin did not affect the induction of COX-2 by IL-1ß, measured at 4 or 48 h.

Conclusions. Ciprofloxacin reduces IL-1ß-induced PGE2 output in tendon-derived cells. The reduction in PGE2 output could modulate various cellular activities of IL-1ß, and may be implicated in fluoroquinolone-induced tendinopathy.

KEY WORDS: Tendon, Fluoroquinolone, Prostaglandin, Interleukin, Cyclooxygenase-2.


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A. N. Corps, R. L. Harrall, V. A. Curry, B. L. Hazleman, and G. P. Riley
Contrasting effects of fluoroquinolone antibiotics on the expression of the collagenases, matrix metalloproteinases (MMP)-1 and -13, in human tendon-derived cells
Rheumatology, December 1, 2005; 44(12): 1514 - 1517.
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