Skip Navigation


Rheumatology Advance Access originally published online on February 17, 2004
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
43/5/569    most recent
keh147v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Wang, C.-R.
Right arrow Articles by Chen, H.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, C.-R.
Right arrow Articles by Chen, H.-C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Rheumatology 2004; 43: 569-573
Rheumatology Vol. 43 No. 5 (c) British Society for Rheumatology 2004; all rights reserved

Basic Science

Up-regulation of XCR1 expression in rheumatoid joints

C.-R. Wang, M.-F. Liu, Y.-H. Huang1 and H.-C. Chen1

Section of Rheumatology and Section of Allergy and Immunology, Department of Internal Medicine, College of Medicine, National Cheng Kung University, Tainan; Taiwan and 1Branch of Molecular Biology, TaiGen Biotechnology, Taipei, Taiwan.

Correspondence to: C.-R. Wang, Section of Rheumatology and Section of Allergy and Immunology, Department of Internal Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan. E-mail: wangcr{at}mail.ncku.edu.tw

Objectives. Chemokine receptor-positive cells play a crucial role in controlling synovitis in rheumatoid arthritis (RA). We studied 16 chemokine receptors of the CC, CXC, CX3C and C families by analysing venous blood and synovial fluid samples and synovial tissues from RA patients.

Methods. Mononuclear cells (MNCs) in paired synovial fluid and venous blood samples from 7 RA patients were studied for the expression of CCR1 to 9, CXCR1 to 5, CX3CR1 and XCR1 by quantitative reverse transcription polymerase chain reaction (RT-PCR). Expression of chemokine receptors on synovial tissues from 9 RA patients were examined by in situ hybridization. Levels of chemokines were measured by enzyme-linked immunosorbant assay.

Results. Higher expression levels of XCR1 and CCR5 in MNCs from synovial fluid, as compared with those from venous blood, were consistently demonstrated in all RA patients (P<0.01). Through in situ hybridization, XCR1 expression was detected in infiltrating MNCs and synoviocytes in synovial tissues. Levels of lymphotactin, the ligand of XCR1, were significantly higher in the joint fluid than those in the paired serum samples (P<0.01).

Conclusions. We found an up-regulation of XCR1 expression in MNCs from the rheumatoid joint, and detected XCR1 expression in infiltrating MNCs in synovial tissues, as well as increased lymphotactin levels in synovial fluid. XCR1-positive cells may play a role in rheumatoid joints.

KEY WORDS: XCR1, Chemokine receptors, Lymphotactin, Chemokines, Rheumatoid arthritis.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.