Rheumatology Advance Access originally published online on June 15, 2004
Rheumatology 2004 43(9):1091-1096; doi:10.1093/rheumatology/keh250
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Rheumatology Vol. 43 No. 9 © British Society for Rheumatology 2004; all rights reserved
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Synovial expression of osteopontin correlates with angiogenesis in juvenile idiopathic arthritis
Second Division of Pediatrics, ‘G. Gaslini’ Institute and Department of Pediatrics, University of Genoa, Genoa, 1 Department of Rheumatology, University of Milan, 2 Department of Pathology, ‘G. Pini’ Institute, Milan and 3 Department of Pathology, ‘G. Gaslini’ Institute, Genoa, Italy.
Correspondence to: M. Gattorno, Second Division of Pediatrics, Rheumatology Unit, ‘G. Gaslini’ Scientific Institute for Children, Largo G. Gaslini 5, 16147, Genoa, Italy. E-mail: marcogattorno{at}ospedale-gaslini.ge.it
Objective. To evaluate the synovial expression of osteopontin (OPN) and its possible correlation with the degree of synovial angiogenesis in human chronic idiopathic arthritis.
Methods. Forty-five patients with active juvenile idiopathic arthritis (JIA) were studied. All patients underwent SF aspiration before steroid injection. A paired plasma sample was collected from 22 JIA patients. Plasma from 15 age-matched healthy subjects was used as control. Plasma and SF were tested by ELISA for OPN and vascular endothelial growth factor (VEGF). Synovial tissue was obtained at synovectomy from 10 JIA patients. Immunohistochemistry was performed according to a standard technique with anti-OPN, anti-CD68, anti-CD31 anti-VEGF and anti-
vß3 antibodies.
Results. OPN levels were significantly higher in SF than in paired plasma samples (P<0.001). The same pattern was observed for VEGF (P<0.001). A positive correlation between OPN and VEGF concentrations was found in SF (r = 0.6, P = 0.001). In synovial tissue, OPN was expressed at the level of the lining and sublining layers with a distribution similar to that observed for VEGF. OPN expression in the lining layer correlated with the number of vessels present in the areas underlying the sublining layer.
Conclusions. Synovial expression of OPN correlates with parameters of angiogenesis in JIA. These data support, in human disease, the possible role of OPN in the vascularization of inflamed synovial tissue, as previously shown in OPN-deficient animal models of arthritis.
KEY WORDS: Osteopontin, Angiogenesis, Integrins, Juvenile idiopathic arthritis
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