Rheumatology Advance Access originally published online on May 11, 2005
Rheumatology 2005 44(8):1021-1025; doi:10.1093/rheumatology/keh672
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Clinical associations of autoantibodies to human muscarinic acetylcholine receptor 3213–228 in primary Sjögren's syndrome
Department of Rheumatology, 1 Department of Physiology and 2 Department of Medical Chemistry, Szeged, University of Szeged, 3 Bay Zoltán Foundation for Applied Research, Institute for Biotechnology, Szeged and 4 Hungarian Academy of Sciences, Clinical Neuroscience Research Group, Pécs, Hungary.
Correspondence to: L. Kovács. E-mail: kovl{at}in1st.szote.u-szeged.hu
Objectives. The authors have previously identified a peptide of the human muscarinic acetylcholine receptor-3 (m3AChR) as a suitable antigen for the immunodetection of antimuscarinic acetylcholine receptor autoantibodies in primary Sjögren's syndrome (pSS). The aim of this study was to assess the clinical correlations and disease specificity of these antibodies.
Methods. Seventy-three pSS, 40 rheumatoid arthritis (RA), 19 systemic lupus erythematosus (SLE), 14 secondary Sjögren's syndrome (sSS) patients, 22 subjects in whom pSS was suspected but in whom the diagnosis not could eventually be established (suspSS) and 40 healthy subjects were investigated. An enzyme-linked immunosorbent assay system developed by the authors using a 16-mer peptide of the m3AChR (m3AChR213–228) in a recombinant fusion peptide form was used as the antigen.
Results. Anti-m3AChR213–228 antibody positivity was observed in 66 (90%) of the pSS patients. The antibody levels correlated positively with the number of extraglandular organ manifestations. Both the mean antibody levels and the occurrence of anti-m3AChR213–228 positivity were significantly higher in pSS than in the comparison groups. The test discriminated the pSS patients from the various comparison groups with specificities of 65, 68, 71 and 50% for RA, SLE, sSS and suspSS, respectively.
Conclusions. The presence of m3AChR213–228 antibodies is a common feature in pSS. Although it is significantly more common in pSS than in the comparison groups, anti-m3AChR213–228 positivity is not exclusive to pSS.
KEY WORDS: Antimuscarinic acetylcholine receptor-3 autoantibody, Disease specificity, Muscarinic acetylcholine receptor-3213–228, Primary Sjögren's syndrome
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