Skip Navigation


Rheumatology Advance Access originally published online on September 11, 2009
Rheumatology 2009 48(11):1447-1450; doi:10.1093/rheumatology/kep262
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
48/11/1447    most recent
kep262v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Clouet, J.
Right arrow Articles by Vinatier, C.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Clouet, J.
Right arrow Articles by Vinatier, C.
Related Collections
Right arrow Osteoarthritis and Cartilage
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Identification of phenotypic discriminating markers for intervertebral disc cells and articular chondrocytes

Johann Clouet1,2, Gaël Grimandi1,2, Marianne Pot-Vaucel1, Martial Masson1, Hakim B. Fellah1, Lydie Guigand3, Yan Cherel3, Eric Bord4, François Rannou5, Pierre Weiss1, Jérôme Guicheux1 and Claire Vinatier1,6

1INSERM (Institut National de la Santé et de la Recherche Médicale), U791, Osteoarticular and Dental Tissue Engineering Group, University of Nantes, 2Pharmacie Centrale, University Hospital of Nantes, 3UMR703-INRA, National Veterinary School, Développement et Pathologie du tissu Musculaire, 4Department of Neurosurgery, University Hospital of Nantes, Nantes, 5INSERM U747, University René Descartes, Paris and 6GRAFTYS SAS, Aix en Provence, France.

Correspondence to: Jérôme Guicheux, INSERM U791, Osteoarticular and Dental Tissue Engineering, University of Nantes, 1-Place Alexis Ricordeau, 44042, Nantes Cedex 1, France. E-mail: jerome.guicheux{at}inserm.fr


  Abstract

Objective. The present study was conducted to improve our knowledge of intervertebral disc (IVD) cell biology by comparing the phenotype of nucleus pulposus (NP) and annulus fibrosus (AF) cells with that of articular chondrocytes (ACs).

Methods. Rabbit cells from NP and AF were isolated and their phenotype was compared with that of AC by real-time PCR analysis of type I (COL1A1), II (COL2A1) and V (COL5A1) collagens, aggrecan transcript (AGC1), matrix Gla protein (MGP) and Htra serine peptidase 1 (Htra1).

Results. Transcript analysis indicated that despite certain similarities, IVD cells exhibit distinct COL2A1/COL1A1 and COL2A1/AGC1 ratios as compared with AC. The expression pattern of COL5A1, MGP and Htra1 makes it possible to define a phenotypic signature for NP and AF cells.

Conclusions. Our study shows that NP and AF cells exhibit a clearly distinguishable phenotype from that of AC. Type V collagen, MGP and HtrA1 could greatly help to discriminate among NP, AF and AC cells.

KEY WORDS: Intervertebral disc, Nucleus pulposus, Annulus fibrosus, Chondrocyte, Phenotype

Submitted 7 April 2009; revised version accepted 22 July 2009.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.