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© 1985 All rights reserved

Differential Expression of Messenger RNAs for Mononuclear Cell Factor from Stimulated Human Monocyte-macrophage Cultures

S. Demczuk, B. Mach and J.-M. Dayer


  Abstract

Lectin-stimulated monocyte-macrophage cultures synthesize mononuclear cell factor (MCF) which upon exposure to human fibroblast and synovial cells induces the release of PGE2 and collagenase MCF shares biological activities with interleukin-1 (1L-1). In a previous report we have shown that fractionated mRNA isolated from cultures stimulated 48 h with phytohaemagglutinin exhibited a sedimentation value of ~ 10-12S and was translated into a protein(s) possessing PGE2- and coUagenase-inducing capabilities. We report here that translation products from fractionated mRNA isolated from 16-h-stimulated culture induce the release of PGE2 (but no detectable release of collagenase) by fibroblasts and synovial cells. This mRNA activity is observed at ~ 16-17S. Unlike the PGE,-inducing material translated from mRNA of 16-h-stimulated cultures, found predominantly in the oocyte incubation buffer, the collagenase- and PGE2-strmulating translation product(s) from 48 h was previously found mostly in the oocytes. Following the stimulation of a rabbit reticulocyte translation system with biologically active mRNA fractions (16-h cultures), immunoprecipitation with antibodies against endogenous pyrogen revealed a protein migrating electrophoretically at Mr ~ 36 kD. These results and results from the literature are complementary in suggesting the existence of several forms of IL-l-like mRNAs coded either by the same or by distinct genes.

KEY WORDS: Mononuclear cell factor, Intrerleukin-1, Messenger RNA, Oocyte translation, PGE2, Collagenase


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