Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (11)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Wedderburn, L. R.
Right arrow Articles by Woo, P.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Wedderburn, L. R.
Right arrow Articles by Woo, P.
Related Collections
Right arrow Vasculitis
Right arrow Vasculitis
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Rheumatology 2001; 40: 1299-1307
© 2001 British Society for Rheumatology

Paediatric Rheumatology

Autologous stem cell transplantation for paediatric-onset polyarteritis nodosa: changes in autoimmune phenotype in the context of reduced diversity of the T- and B-cell repertoires, and evidence for reversion from the CD45RO+ to RA+ phenotype

Paediatric Rheumatology/Series Editor: P. Woo

L. R. Wedderburn, R. Jeffery, H. White1, A. Patel, H. Varsani, D. Linch2, K. Murray and P. Woo

Paediatric Rheumatology Unit, Institute of Child Health and Department of Molecular Pathology, University College London,
1 Molecular Immunology Unit, Institute of Child Health and
2 Department of Haematology, University College London Hospital, London, UK

Abstract

We have studied immune reconstitution in a patient with paediatric-onset polyarteritis nodosa treated with high-dose immunosuppressive agents followed by stem cell rescue. The patient developed several new autoimmune phenomena over the 18 months after immunosuppression and stem cell rescue. Flow cytometry, reverse transcription–polymerase chain reaction (RT-PCR) heteroduplex and isotype-specific RT-PCR analysis of immunoglobulin expression showed that the T- and B-cell repertoires were highly restricted in the first few months after treatment. The dominant T-cell clones seen after reconstitution were persistently expanded, were different from those which could be demonstrated before autologous stem cell transplantation, and were in the CD8+ population. Our data also show that 12 months after treatment these expanded T-cell clones were within the CD45RA+ population, suggesting that reversion from the CD45RO+ to the CD45RA+ phenotype had occurred in vivo.

KEY WORDS: T-cell receptor, Repertoire, CD45, Memory, Autologous stem cell transplantation, Vasculitis, Immune reconstitution, High-dose immunosuppression.

Notes

Corresponding author: L. R. Wedderburn, Rheumatology Unit, Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Arch. Dis. Child.Home page
L R Wedderburn, M Abinun, P Palmer, and H E Foster
Autologous haematopoietic stem cell transplantation in juvenile idiopathic arthritis
Arch. Dis. Child., March 1, 2003; 88(3): 201 - 205.
[Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.