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Rheumatology Advance Access originally published online on April 24, 2009
Rheumatology 2009 48(6):632-637; doi:10.1093/rheumatology/kep070
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© 2009 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Redefining the Scl-70 indirect immunofluorescence pattern: autoantibodies to DNA topoisomerase I yield a specific compound immunofluorescence pattern

Alessandra Dellavance1,2, Cristiane Gallindo1, Mariana Guanaes Soares2, Neusa Pereira da Silva2, Renato Arruda Mortara3 and Luís Eduardo Coelho Andrade1,2

1Fleury Medicine and Health, 2Rheumatology Division and 3Microbiology, Immunology and Parasitology Division, Universidade Federal de São Paulo, São Paulo, Brazil.

Correspondence to: Luís Eduardo Coelho Andrade, Rheumatology Division, Universidade Federal de São Paulo, Rua Botucatu 740 3° andar, 04023-062 São Paulo, Brazil. E-mail: luis.andrade{at}unifesp.br


  Abstract

Objectives. To report on a novel IIF pattern specifically associated with antibodies to DNA topo I.

Methods. A novel compound IF pattern, designated Scl-70 pattern, was characterized in routine ANA-HEp-2 IIF screening. Within the last 3 years, all serum samples presenting the Scl-70 pattern at the ANA-HEp2 IIF screening were tested for anti-topo I reactivity. Conversely, 16 serum samples with known anti-topo I reactivity and affinity-purified anti-topo I antibody preparations were tested for the Scl-70 pattern.

Results. The Scl-70 pattern comprised the staining of five cellular regions: nucleus, nucleolus and cytoplasm in interphase cells; nucleolar organizing region (NOR) and chromosomes in mitotic cells. All 81 serum samples selected as Scl-70 pattern reacted with topo I. All 16 anti-topo I samples and antibody preparations reproduced the Scl-70 pattern. This compound IF pattern was consistently observed in different commercial HEp-2 cell slides and in home-made slides with HEp-2 cells and human fibroblasts fixed with alternative protocols. Double IIF experiments demonstrated the co-localization of topo I and human upstream binding factor at the NOR.

Conclusions. The Scl-70 pattern belongs to the group of compound IF patterns that hold strong association with the respective autoantibody specificities, such as that observed with centromere protein F (CENP-F) and nuclear mitotic apparatus-1 (NuMA-1) protein. The identification of the Scl-70 pattern at routine ANA-HEp-2 IIF screening may lead to implementation of specific tests for the identification of anti-topo I antibodies. In addition, the Scl-70 pattern outlines cellular domains other than those previously reported for topo I, which is of interest for further understanding the roles of this enzyme in cell biology.

KEY WORDS: Anti-nuclear antibodies, DNA topoisomerase I, Scl-70, Autoantibodies, Systemic sclerosis

Submitted 6 December 2008; revised version accepted 6 March 2009.
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