Rheumatology Advance Access published online on December 1, 2003
Rheumatology, doi:10.1093/rheumatology/keh061
Rheumatology © British Society for Rheumatology 2003; all rights reserved
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Original Papers
1 Departamento de Biología Celular, Facultad de Biología, Universidad Complutense de Madrid, Madrid, Spain
* Corresponding author. E-mail: gomariz{at}bio.ucm.es.
Received 18 June 2003
; accepted 24 September 2003
Objective. Vasoactive intestinal peptide (VIP) has demonstrated beneficial effects in several murine models of immune-mediated inflammation by inhibiting both the inflammatory and the autoimmune components of the disease. We investigate its potential to modulate the release of proinflammatory cytokines and chemokines by human synovial cells from patients with rheumatoid arthritis (RA). Methods. Fresh suspensions of synovial tissue cells (STC) or cultured fibroblast-like synoviocytes (FLS) were obtained from patients with RA or osteoarthritis (OA). The effects of VIP on basal or tumour necrosis factor Results. VIP at 10 nm down-regulated chemokine production by STC and FLS from RA and OA patients. VIP also down-regulated the expression of mRNAs for CCL2, CXCL8 and IL-6. The effects of VIP were more clearly detected in RA samples and after stimulation with TNF- Conclusion. Our observations confirm that the proposed anti-inflammatory actions of VIP in murine models also apply to human synovial cells ex vivo. Further studies are encouraged to evaluate the use of VIP as a potential therapy for chronic inflammatory joint diseases.
Key words: VIP, Rheumatoid arthritis, CXCL8, CCL2, TNF-Vasoactive intestinal peptide modulates proinflammatory mediator synthesis in osteoarthritic and rheumatoid synovial cells
2 Servicio de Reumatología y Unidad de Investigación, Hospital 12 de Octubre, Madrid, Spain
(TNF-)-stimulated production of CCL2 (MCP-1, monocyte chemotactic protein 1), CXCL8 [interleukin (IL)-8], IL-6 and TNF- were studied by specific ELISAs (enzyme-linked immunosorbent assays). The mRNAs for CCL2, CXCL8 and IL-6 in FLS were analysed by real-time reverse transcription-polymerase chain reaction.., IL-6, Synoviocytes.
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