Rheumatology Advance Access published online on May 18, 2004
Rheumatology, doi:10.1093/rheumatology/keh195
Rheumatology © British Society for Rheumatology 2004; all rights reserved
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1 Division of Rheumatology, Saga Medical School, Saga, Japan
* To whom correspondence should be addressed. E-mail: koarada{at}post.saga-med.ac.jp.
Objectives. To investigate the relationship between the production of Th1/Th2 cytokines and cell kinetics, cell division and proliferation in patients with Behçet's disease (BD). Methods. Peripheral venous blood was drawn from patients with BD (n = 24; 10 patients with active and 14 patients with inactive BD) and normal subjects (n = 22). Peripheral blood mononuclear cells were separated immediately and were cultured with concanavalin A (Con A) followed by phorbol 12-myristate 13-acetate and ionomycin (PMA+Ion). Intracellular cytokine production of interferon- Results. In active BD, enhanced entry into the Th1 response effector pathway of CD4+ T cells was observed after stimulation with Con A followed by PMA+Ion. Analysis of CD4+ T cells at an identical cell division number in response to Con A followed by PMA+Ion revealed that IFN- Conclusions. Cell kinetics play a crucial role in Th1 cell differentiation and pathophysiology in BD. KEY WORDS:
Behçet's disease, Cell division, Th1/Th2.
Revised March 16, 2004
Original Papers
Increased entry of CD4+ T cells into the Th1 cytokine effector pathway during T-cell division following stimulation in Behçet's disease
2 Clinical Nursing, Saga Medical School, Saga, Japan
Abstract 
(IFN-) (Th1) and IL-4 (Th2) in CD4+ T cells was determined by flow cytometry. Furthermore, CD4+ T cells labelled with CFSE [5 (and 6) carboxyfluorescein diacretate, succinimidyl ester] were stimulated and the cells were analysed for entry into the cytokine production effector pathway during cell division in active BD and normal subjects.-producing cells were increased in active BD patients compared with normal subjects. These results suggest that the Th1 response of dividing CD4+ T cells is predominantly operating in active BD. Dividing CD4+ T cells stimulated with Con A followed by PMA+Ion showed a phenotype of activated effector memory T cells (CD45RAlow, CD45RO+, CD69high).
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