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Rheumatology Advance Access published online on July 20, 2004
Rheumatology, doi:10.1093/rheumatology/keh323
Rheumatology © British Society for Rheumatology 2004; all rights reserved
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Received April 1, 2004
Accepted June 21, 2004

Original Papers

Different cytokine profiles in patients with chronic and acute reactive arthritis

I. Butrimiene 1*, S. Jarmalaite 2, J. Ranceva 1, A. Venalis 1, L. Jasiuleviciute 1, A. Zvirbliene 3

1 Institute of Experimental and Clinical Medicine, Vilnius University, Lithuania
2 Laboratory of Ecological Genetics, Vilnius University, Lithuania
3 Laboratory of Immunology, Institute of Biotechnology, Lithuania

* To whom correspondence should be addressed. E-mail: irena.butrimiene{at}santa.lt.


  Abstract

Objective. Analysis of cytokine production in patients with acute and chronic reactive arthritis (AcReA/ChrReA) in order to search for new treatment possibilities.

Methods. Cytokine production by peripheral blood and synovial fluid mononuclear cells (PBMCs/SFMCs) of 28 patients with AcReA, 27 patients with ChrReA, 26 patients with rheumatoid arthritis (RA) and 31 healthy controls was analysed by enzyme-linked immunosorbent assay (ELISA) and flow-cytometry. Production of tumour necrosis factor-alpha (TNF-{alpha}), interferon-gamma (IFN-{gamma}) and interleukin (IL)-10 was measured by ELISA, while the percentages of TNF-{alpha}-, IFN-{gamma}- and IL-4-positive CD3+ cells were determined in the same groups of patients and healthy subjects using flow cytometry.

Results. Spontaneous TNF-{alpha} production observed in PBMCs of ChrReA, but not of AcReA, patients was significantly higher (P<0.001) than in healthy controls. The percentages of TNF-{alpha}-positive CD3+ blood cells in ChrReA exceeded that of RA patients and healthy controls (P<0.05 and P<0.001, respectively). Also, the percentages of IFN-{gamma}-positive CD3+ cells were significantly higher in peripheral blood and synovial fluid of ChrReA patients (P<0.05 and P<0.05, respectively) as compared with AcReA. In ChrReA spontaneous IL-10 production in PBMCs was similar to that observed in healthy controls, while in RA and AcReA the production of IL-10 was significantly increased (P<0.05 and P<0.05, respectively). IL-4 production was low in all study groups with no significant differences detected.

Conclusions. High production of TNF-{alpha} and IFN-{gamma} detected in ChrReA supports the possible use of anti-TNF-{alpha} treatment in ChrReA.

Keywords: Reactive arthritis; Cytokines; TNF-{alpha}; IFN-{gamma}.
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