Skip Navigation



Rheumatology Advance Access published online on June 7, 2005
Rheumatology, doi:10.1093/rheumatology/keh697
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
44/9/1136    most recent
keh697v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Sánchez, E.
Right arrow Articles by Martín, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sánchez, E.
Right arrow Articles by Martín, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org
Received March 9, 2005
Accepted April 29, 2005

Concise Report

Interleukin 12 (IL12B), interleukin 12 receptor (IL12RB1) and interleukin 23 (IL23A) gene polymorphism in systemic lupus erythematosus

E. Sánchez 1, S. Morales 1, L. Paco 2, M. A. López-Nevot 2, C. Hidalgo 3, J. Jiménez-Alonso 3, B. Torres 2, M. A. González-Gay 4, J. L. Callejas 5, N. Ortego-Centeno 5, J. Sánchez-Roman 6, M. F. González-Escribano 2, and J. Martín 1*

1 Instituto de Parasitología y Biomedicina López-Neyra, CSIC, Spain
2 Servicio de Inmunología, Hospital Virgen del Rocío, Seville, Spain
3 Servicio de Medicina Interna, Hospital Virgen de las Nieves, Granada, Spain
4 Servicio de Reumatología, Hospital Xeral-Calde, Lugo, Spain
5 Servicio de Medicina Interna, Hospital Clínico San Cecilio, Granada, Spain
6 Servicio de Medicina Interna, Hospital Virgen del Rocío, Seville, Spain

* To whom correspondence should be addressed.
J. Martín, E-mail: martin{at}ipb.csic.es


  Abstract

Objective. The aim of this study was to assess the possible association between the interleukin-12B (IL12B) and interleukin-12 receptor beta 1 (IL12RB1) gene polymorphisms with systemic lupus erythematosus (SLE). In addition, we have undertaken a systematic search for genetic variants of interleukin 23 (IL23A).

Methods. The study was conducted on 559 SLE patients and 603 ethnically matched healthy controls. Genotyping of the IL12B [IL12Bpro and IL12B 3' untranslated region (UTR)] and IL12RB1 (641A->G, 1094T->C and 1132G->C) polymorphisms was performed with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and PCR-fluorescent methods, whereas IL23A genetic variants were realized with direct sequencing.

Results. No statistically significant differences in the distribution of the IL12B and the IL12RB1 genotypes and alleles were observed when comparing SLE patients and control subjects. Additionally, no differences in the genotype and allele distribution were found when SLE patients were stratified according to the presence or absence of lupus nephritis. Despite an extensive analysis in 30 individuals, variations located in the exons and in the 5' and 3' UTR regions of IL23A gene were not found in any case.

Conclusions. These results suggest that polymorphisms located in IL12B, IL12RB1 and IL23A genes may not play a relevant role in the susceptibility or severity of SLE in the Spanish population.

Keywords: Systemic lupus erythematosus (SLE); Interleukin-12 (IL-12); Interleukin-12 receptor (IL-12R); Interleukin-23 (IL-23); Polymorphism; Susceptibility.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.