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Rheumatology Advance Access published online on June 12, 2006
Rheumatology, doi:10.1093/rheumatology/kel159
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© The Author 2006. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org
Received December 15, 2005
Accepted April 4, 2006

Original Papers

Up-regulation of IL-23p19 expression in rheumatoid arthritis synovial fibroblasts by IL-17 through PI3-kinase-, NF-{kappa}B- and p38 MAPK-dependent signalling pathways

H.-R. Kim 1, M.-L. Cho 2, K.-W. Kim 2, J.-Y. Juhn 2, S.-Y. Hwang 3, C.-H. Yoon 2, S.-H. Park 2, S.-H. Lee 1, and H.-Y. Kim 2 *

1 Department of Internal Medicine, School of Medicine, Konkuk University, Seoul, Korea
2 Rheumatism Research Center, Catholic Institutes of Medical Science, The Catholic University of Korea, Seoul, Korea
3 Graduate School of Biology and Information Technology, Institute of Genetic Engineering, Hankyung National University, Ansung, Kyonggi-Do, Korea

* To whom correspondence should be addressed.
H.-Y. Kim, E-mail: ho{at}catholic.ac.kr


  Abstract

Objective. To investigate the expression of interleukin (IL)-23p19 in human rheumatoid arthritis (RA) synovial fibroblasts and its up-regulation by IL-17 stimulation, and to define the signal pathways involved in the regulation of IL-23p19 expression in RA synovial fibroblasts.

Methods. Synovial fluid (SF) and serum levels of IL-23p19 in RA were determined by enzyme-linked immunosorbent assays. The levels of IL-23p19 mRNA and protein were measured after the RA synovial fibroblasts were treated with recombinant human IL-17 and various inhibitors of intracellular signal pathway molecules using reverse transcription (RT) polymerase chain reaction (PCR), real-time PCR and western blotting.

Results. Levels of IL-23p19 in the sera and SF were much higher in RA patients than in osteoarthritis patients or healthy controls. The expression of IL-23p19 mRNA and protein was enhanced in RA synovial fibroblasts by IL-17 stimulation. Such effects of IL-17 were completely blocked by inhibitors of phosphatidylinositol (PI)-kinase/Akt, nuclear factor (NF)-{kappa}B and p38 mitogen-activated protein kinase (MAPK). In accordance with the expression of IL-23p19, the phosphorylation of I{kappa}B, Akt and p38 MAPK in synovial fibroblasts also increased after IL-17 stimulation.

Conclusion. IL-23p19 is over-expressed in RA synovial fibroblasts and IL-17 appears to up-regulate the expression of IL-23p19 in RA synovial fibroblasts via PI3-kinase/Akt, NF-{kappa}B- and p38-MAPK-mediated pathways. These results suggest that a disruption of interaction between IL-17 and IL-23p19 may provide a new therapeutic approach in the treatment of RA.

Keywords: Rheumatoid arthritis; Synovial fibroblast; IL-23; IL-17.
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