Rheumatology Advance Access published online on November 3, 2006
Rheumatology, doi:10.1093/rheumatology/kel346
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Second Division of Pediatrics, ‘G. Gaslini’ Institute for Children and University of Genoa, Genoa, Italy
* To whom correspondence should be addressed. Objectives. Recent laboratory and clinical data suggest that two prototype autoimmune diseases, systemic lupus erythematosus and rheumatoid arthritis are mainly driven by distinct cytokines, interferon (IFN)- Methods. Peripheral blood (PB) and synovial fluid (SF) mononuclear cells (MNCs) (n = 25 each) from JIA patients with active disease were studied. IPCs were identified as BCDA-2+CD123+HLA-DR+CD45RA+ cells, and dendritic cells (DCs) as CD11c+CD14-/lowlin- cells by flow cytometry. IPCs and DCs were analysed for Toll-like receptor-7 and -9 mRNA expression by real-time polymerase chain reaction. IFN- Results. IPCs were enriched in SF MNCs compared with PB MNCs in all JIA patients. Influenza-induced, but no spontaneous IFN- Conclusions. These data suggest that besides TNF-
Received December 16, 2005
Accepted September 8, 2006
Original Papers
Distinct expression pattern of IFN-
M. Gattorno 1 *, L. Chicha 2, A. Gregorio 1, F. Ferlito 1, F. Rossi 1, D. Jarrossay 2, A. Lanzavecchia 2, A. Martini 1, and M. G. Manz 2
and TNF- in juvenile idiopathic arthritis synovial tissue
2 Institute of Research in Biomedicine, Bellinzona, Switzerland
M. Gattorno, E-mail: marcogattorno{at}ospedale-gaslini.ge.it
Abstract 
and tumour necrosis factor (TNF)-, respectively. We here investigated the presence and characteristics of natural type I IFN-producing cells (IPCs), as well as IFN- and TNF- expression at sites of inflammation in juvenile idiopathic arthritis (JIA). was measured by enzyme-linked immunosorbent assay in serum, SF and in supernatants of influenza virus-infected, cultured IPCs. Synovial tissues of n = 6 additional JIA patients were analysed by immunohistochemistry using mAbs against CD123, IFN-, TNF-, CD3, CD19 and CD138. release was detected from SF IPCs, and serum and SF IFN- levels were not elevated. Nonetheless, in synovial tissue IFN- producing cells accumulated at inflammatory lymph-follicular-like structures, while TNF- producing cells were mostly found at the lining and sublining layers.-expressing cells, IFN--producing IPCs are involved in initiation, maintenance or regulation of the inflammatory response in JIA.; Plasmacytoid cells; Dendritic cells; Juvenile idiopathic arthritis.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. M. Manzaneque, F. M. Vera, F. M. Rodriguez, G. J. Garcia, L. Leyva, and M. J. Blanca Serum Cytokines, Mood and Sleep after a Qigong Program: Is Qigong an Effective Psychobiological Tool? J Health Psychol, January 1, 2009; 14(1): 60 - 67. [Abstract] [PDF]
|
|
|
|
 |
|
 J. C. Miller, Y. Ma, J. Bian, K. C. F. Sheehan, J. F. Zachary, J. H. Weis, R. D. Schreiber, and J. J. Weis A Critical Role for Type I IFN in Arthritis Development following Borrelia burgdorferi Infection of Mice J. Immunol., December 15, 2008; 181(12): 8492 - 8503. [Abstract] [Full Text] [PDF]
|
|