Rheumatology Advance Access published online on October 15, 2007
Rheumatology, doi:10.1093/rheumatology/kem235
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The polymorphism -863C/A in tumour necrosis factor-
gene contributes an independent association to gout
Department of Public Health, Faculty of Medicine, College of Medicine, 1Institute of Public Health, College of Health Science, Kaohsiung Medical University and 2Division of Rheumatology, Allergy and Immunology, Department of Internal Medicine, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan
Correspondence to:
S.-J. Chang, PhD, Department of Public Health, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 807, Taiwan. E-mail: changsj{at}kmu.edu.tw
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Abstract |
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Objective. To investigate the associations between polymorphisms in the promoter of the tumour necrosis factor-
(TNF-) gene and gout.
Methods. The polymorphisms -308G/A and -863C/A in the TNF- gene were determined in 106 gout patients and 159 healthy controls among male Taiwanese using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. The biochemical markers, including Glutamic-oxaloacetic transaminase (GOT), Glutamic-pyruvic transaminase (GPT), uric acid, creatinine, total cholesterol (TC), triglycerides (TG), body mass index (BMI) and hypertension, as well as alcohol consumption were measured.
Results. The gout patients had 9.43% (10/106) with genotype AA at polymorphism -863C/A showing a significantly higher fraction than controls (0.63%; 1/159, P < 0.001). The crude results also showed that the gout patients had significantly higher portions of abnormal GOT, GPT, creatinine, TC, TG, alcohol consumption, hypertension and hyperuricaemia than controls (P < 0.05), but the -308G/A, BMI and genotype CA at -863C/A did not show the same significant difference (P > 0.05). After adjustment by a stepwise logistic regression method, the hyperuricaemia, creatinine, GPT, TG and alcohol consumption as well as genotype AA at polymorphism -863C/A were found to be significantly associated with gout.
Conclusion. The genotype AA at polymorphism -863C/A in a recessive model showed a significant association with developing gout independent of hyperuricaemia, abnormal creatinine, higher TG, GPT and alcohol consumption.
KEY WORDS: Gout, Tumour necrosis factor, Hyperuricaemia, Creatinine
Submitted 11 May 2007;
revised version accepted 3 August 2007.
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